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KMID : 0900919990230010019
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Korean journal of Animal Reproduction
1999 Volume.23 No. 1 p.19 ~ p.27
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Effect of Parthenogenesis of Mouse Oocyte following Intracytoplasmic Injection with Human Sperm Extract
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Jeon Eun-Sook
Park Chang-Sik
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Abstract
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This study was carried out to investigate the effect of intracytoplasmic injection of Ca^{2＋} and human sperm extract on the parthenogenetic activation of mouse oocytes. The results obtained were as follows: 1. The mouse oocytes were injected with 10 pl of PBS medium containing 0, 1.7 and 5 mM calcium concentrations, respectively. Activation rate of the oocytes with formation of pronuc1ei and extrusion of the second polar bodies was 14.5, 9.8 and 14.9% at the above calcium concentrations, respectively. There were no significant differences in the activation rates among the calcium concentrations. 2. The mouse oocytes were injected with 10 pl of non-heated human sperm extract, and cultured for 12~15 h in the PBS media with the 0, 1.7 and 5 mM calcium concentrations, respectively. Activation rate(51.8%) of the oocytes at the 1.7 mM calcium concentration was significantly higher than those at the 0 and 5 mM calcium concentrations. 3. The mouse oocytes were injected with 10 pl of heated human sperm extract, and cultured for 12~15 h in the PBS media with the 0, 1. 7 and 5 mM calcium concentrations, respectively. No significant differences were found in the activation rates (11.8~17.0%) among the calcium concentrations. 4. The mouse oocytes were injected with 10 pl of PBS medium, non-heated sperm extract and heated sperm extract, and cultured for 12~15 h in the PBS media with 1.7 mM calcium concentrations, respectively. Activation rate (54.5%) of the oocytes injected with the non-heated sperm extract was highest. There were significant differences in the activation rates among the above injection materials (P＜0.05). 5. The mouse oocytes were injected with 10 pl of 1 and 6 days old non-heated sperm extracts, and cultured for 12＜15 h in the PBS media with 1.7 mM calcium concentrations, respectively. Activation rate(60.0%) of the oocytes injected with 1 days old sperm extract was significantly higher than that (11.1%) injected with 6 days old sperm extract. The results obtained in this study suggest that non-heated human sperm extract may contain sperm-associated oocyte-activating factor such as oscillin.
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KEYWORD
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Parthenogenesis, Sperm extract, Activation, Oscillin
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